Fig. 5: Detecting mutations in a synthetic TP53 exon using CyclomicsSeq.
From: Accurate detection of circulating tumor DNA using nanopore consensus sequencing
a Box plots (center line = median; box limits = 25th and 75th percentiles; whiskers = 1.5× interquartile range; data points = outliers) depicting 1 - single-nucleotide false positive (snFP) rate in the insert (17:7577010–7577150 in GRCh37) per number of repeats for 8 PCR and 3 PCR-free inserts. b Calls in WT and MUT at the three mutant positions in TP53. Data points represent single reads. Colors indicate base call in the consensus-called read. Numbers >20 reads are shown, of which true negatives and true positives are indicated in white. c Observed mutation rate in WT and mixed WT/MUT at the three mutant positions in TP53. Expected mutation rates are 0.000 in WT and 0.001 in mixed WT/MUT.
