Fig. 4: MiRNAs expression in synaptosome in AD and healthy state.

a Hierarchical clustering and heatmap of significantly deregulated miRNAs in synaptosome in AD and unaffected controls. (red color intensity showed the miRNAs upregulation and blue color intensity showed the miRNAs downregulation) b Total numbers of miRNAs deregulated in AD synaptosome vs UC synaptosome. (grayscale bar—total number of miRNAs; red scale bar—upregulated miRNAs; green scale bar—downregulated miRNAs). c Pi diagram showed the total miRNAs pool distribution and percentage of miRNAs population changed in AD synaptosome vs UC synaptosome. d qRT-PCR-based validation analysis of significantly deregulated miRNAs in AD (n = 27) and UC (n = 15) synaptosome. MiRNAs expression was quantified in terms of fold changes in AD synaptosome relative to UC synaptosome. Each circle dot represents one sample. e Multiple comparison analysis of synaptosomal miRNAs fold changes with Braak stages 2/3, Braak stages 4/5 and Braak stages 6 of AD samples. (**P < 0.01, ***P < 0.001, ****P < 0.0001). f Immunoblotting analysis of miRNAs biogenesis proteins (Ago2, Drosha, and Dicer) in the cytosol and synaptosomal of UC samples (n = 4). g Densitometry analysis of Ago2, Drosha and Dicer in cytosol relative to synaptosomes of UC samples. All blots are driven from the same experiment and were proceeded parallelly (f). Values in the bar diagrams are mean ± SEM and error bars are equivalent throughout the figure (d, g).