Fig. 2: Globin reduction increases sequencing sensitivity.
a Stacked bars showing globin gene reads in pre- or post-GR samples. Hemoglobin genes are shown in the legends beside the plots. b Bar plots showing the number of mRNA reads for samples before and after GR. The red segments represent reads from globin genes. c Boxplots showing the NGRI scores in the pre- and post-GR groups. Box limits indicate the range of the central 50% of the data with the central line marking the median value. Light gray dots show the NGRI scores in each group. Dashed lines connect the NGRI scores for pre- and post-GR samples from the same donor. A paired t-test was used for comparison. d Stacked bars showing total counts of aligned genes in pre- and post-GR samples. Count categories are genes with 5–20 counts, 20–100 counts, 100–500 counts, 500–2000 counts, 2000–5000 counts, 5000–10000 counts, or >10000 counts per sample. e Sample-level scatterplots comparing log2 normalized counts before and after GR, with post-GR samples plotted along the x-axis and pre-GR samples plotted along the y-axis. The red lines have slope = 1 and pass through the origin, and the red dots represent globin genes. Pearson’s correlation coefficient (R) for each scatterplot is shown. f Bar plots showing the number of DEGs surviving FDR ≤ 0.05 before and after GR. The red segments represent upregulated genes, while blue represents downregulated genes.
