Fig. 6: Expression comparison of selected genes using TCGA-GBM transcriptome profiling data and R2 genomics RNA profiling data.

a Expression of 98 genes from the 105 DEGs shown in main Fig. 4 in TCGA-GBM tissue data (22 genes are not available in TCGA). Potential function or pathway annotations are labeled on the left side of the heatmap. Genes in the GBM-Dx panel are labeled in hot pink. b The R2 genomics database was queried for eight genes (in the GBM-Dx panel) comparing normal brain data and GBM tissue data. The GBM patient cohorts used are “N B (172)”, representing the N Brain 172 (Berchtold) dataset, “N H (44)”, representing the N Brain 44 (Harris) dataset as the normal control group, “T P (46)”, representing the T Glioblastoma 46 (Pfister) dataset, “T L (70)”, representing the T Glioblastoma 70 (Loeffler) dataset, and “T H (84)”, representing the T Glioblastoma 84 (Hegi) dataset as the GBM tumor group. Boxplots were generated via R2: MegaSampler. Box limits indicate the range of the central 50% of the data (from 75th to 25th quantiles) with the central line marking the median value. The upper and lower whiskers are the maximum and minimum values of the data that are within 1.5 times the interquartile range over the 75th and 25th percentiles. P-values were computed via one way analysis of variance (ANOVA). Datasets of normal (N) samples are labeled in gray, and datasets of glioblastoma tumor (T) samples are labeled in salmon under the x-axis. The numbers in brackets represent the sample sizes in the datasets.