Fig. 4: Flow cytometric analysis of T cell subsets.
From: Pre-T cell receptor-α immunodeficiency detected exclusively using whole genome sequencing
A Patient CD8+ T cell numbers are relatively reduced compared to CD4+, resulting in a CD4:CD8 ratio of 4.5-5:1. B The CD4+ T cell CD45RA+CD28+ naive cell population is significantly low for age. C Increased CD45RA+CD28+CD95+ stem like T cells, but (D) normal CD31 expression by naive cells. E Maintenance of CCR7+ central memory (Tcm) and CCR7- transitional memory (Ttm) subpopulations. F High levels of ICOS-1 expression by CD4+ memory T cells. G Expression of IL-7R (CD127) was reduced in patient CD8+ cells, particularly within the CD45RA- memory T cell populations, including CCR7+ central and CCR7- transitional memory subsets (H).
