Fig. 4

Distribution of gene functional categories differentially expressed during normal gravity growth relative to simulated microgravity. RNA isolated from 8 h normal gravity and simulated microgravity S. mutans HARV cultures (n = 3 independent experiments per growth condition) was subjected to RNA-seq transcriptome profiling as described in “Materials and methods”. Differential expression and statistical analysis was carried out using a cutoff FDR-adjusted p value of 0.05. Of this dataset, those genes with a log₂ (fold-change) value of at least ±1.0 were grouped by functional classification based on DAVID gene functional categorization analysis, NCBI gene annotations and KEGG pathway analysis. Total number of up-regulated genes (log₂ fold-change ≥1.0, black bars) = 94, total number of down-regulated genes (log₂ fold-change ≤ −1.0, gray bars) = 153