Fig. 2
An insertion of the transposon IS1 to the left of position 1,905,401 in the forward orientation (+) is predicted (coordinate as annotated in NC_000913.2). At the point of insertion, it duplicates nine base pairs (1905401-1905409). Thus, the sequence goes up to 1,905,409 on the left side and then goes into the IS1 element, which is inverted, and then comes out at 1,905,401 and continues to higher coordinates. All of this occurs within the “cspC” gene, thereby likely making it dysfunctional. Likewise, transposon insertions have been predicted for positions 3,580,708 (four base pairs) and 4,540,601 (nine base pairs), disrupting the genes yhhZ (conserved protein) and fimE (tyrosine recombinase/inversion of on/off regulator of fimA), respectively. The JC (New junction) evidence thus shows the two new junctions (one on each side) that the mutations were predicted from. Two other transposon insertions occur in intergenic regions and may not have any impact on the actual genome itself. This figure was prepared by Quyen Tran
