Table 1 Mutations found in E. coli MG1655 (lac plus) after 1000 generations of LSMMG
Position | Type of change | Mutation type | Annotation | Gene | Usual product | Function |
|---|---|---|---|---|---|---|
54,033 | G→T | Base change | Q224K (CAG→AAG) | surA a ← | Periplasmic peptidyl-prolyl isomerase | Outer membrane protein folding |
326,446 | C→T | Base change | G9D (GGT→GAT) | betA ← | Choline dehydrogenase (flavoprotein) | Protection against osmotic stress |
3,015,315 | G→A | Base change | G412R (GGA→AGA) | ygfK → | Possible oxidoreductase, Fe—S subunit | Putative selenate reductase |
4,031,240 | G→A | Base change | G25R (GGG→AGG) | trkH → | Potassium transporter | Transport |
4,546,957 | G→C | Base change | V43L (GTA→CTA) | fimH → | Minor type 1 fimbriae subunit | d-Mannose-specific adhesin |
547,694 | A→G | Base change | Pseudogene (139/252 nt) | ylbE c ← | C‑terminal fragment (pseudogene) | Two mutations make ORF |
547,831 | +G | Base insertion/addition | Pseudogene (2/252 nt) | ylbE c ← | C‑terminal fragment (pseudogene) | unknown function |
169,483 | Δ1 bp | Base deletion | Coding (2000/2244 nt) | fhuA → | ferrichrome outer membrane transporter | Outer membrane transport |
1,976,527 | Δ776 bp | Base deletion | Intergenic | insB–insA | – | – |
1,050,465 | T→A | Base change | Intergenic (−67/−219) | cspH←/→cspG | – | – |
3,957,957 | C→T | Base change | Intergenic (−121/+78) | ppiC←/←yifN | – | – |
1,905,401 | IS1 (+)+9 bp | Transposon MRb | coding (51–59/210 nt) | cspC ← | Deletes CspA-family stress protein | Stress |
1,977,510 | IS5 (+)+4 bp | Transposon MR | intergenic (−271/−264) | insA←/→uspC | – | |
2,175,275 | IS1 (+)+9 bp | Transposon MR | intergenic (−49/+251) | gatY←/←fbaB | – | |
3,580,708 | IS5 (–)+4 bp | Transposon MR | coding (823–826/1179 nt) | yhhZ → | Deletes conserved hypothetical protein | |
4,540,601 | IS1 (+)+9 bp | Transposon MR | coding (542–550/597 nt) | fimE → | Deletes tyrosine recombinase | Inversion of on/off fimA regulator |
