Fig. 2

Spaceflight impacts expression of genes involved in mechanotransduction and cytoskeleton maintenance. Integrin and mechanical signaling impact Hippo activity and small RhoGTPases, which function along the Wnt planar cell polarity pathway a. YAP1 expression was significantly reduced in neonatal CPCs and upregulated in adult CPCs b. RHOA, a small RhoGTPase, was expressed at significantly lower levels in neonatal c, but not adult d, CPCs. Accordingly, cytoskeletal gene expression was significantly upregulated in neonatal e, but not adult f, CPCs. n = 3 biological replicates for all gene expression data, except YAP1 (n = 6 measures of three pooled clones). Data are reported as the mean ± SEM, *P < 0.05, **P < 0.01