Fig. 3
From: Single-cell analysis reveals individual spore responses to simulated space vacuum
Germination, outgrowth, elongation, and growth of individual PS533 spores without or with high vacuum treatment. Spores with or without a 10 day high vacuum treatment were incubated on an LB medium agar pad on a microscope coverslip at 37 °C and spore and cell images were obtained by DIC microscopy as described in Methods. a, b Cell images at various times. In the 0.01 min images, the red arrows in a and b indicate spores that germinated and ultimately grew, the black arrow in b indicates a spore that never germinated, and the blue arrow in (b) indicates a spore which germinated but did not grow. c, e Intensities of bright-field DIC images at various culture times; and d, f spore/cell length vs culture time
