Fig. 6
From: Single-cell analysis reveals individual spore responses to simulated space vacuum
High vacuum killing of B. subtilis spores with defects in spore structural components a, b or DNA repair (c). Spores of strain PS832 and its isogenic derivatives were exposed to high vacuum and spore survival was determined as described in Methods. The spore surviving fraction was determined from the ratio of N/N0, with N0 the number of CFU of the untreated spores (spores stored dry in a laboratory desiccator) and N the high vacuum-treated spores. Values were analyzed in multigroup pairwise combinations, and differences with P values of <0.05 were considered statistically significant. Gray bars, laboratory storage; white bars, high vacuum-treated spores. Plus, spore resistance to laboratory storage significantly different than the wild type; astereisk, spore resistance to high vacuum significantly different than the wild type
