Fig. 7
From: Single-cell analysis reveals individual spore responses to simulated space vacuum
Germination a, c and growth b, d of multiple individual untreated or high vacuum-treated B. subtilis PS533 (wild type) a, b or PS578 (α−β−) c–f spores. B. subtilis PS533 or PS578 spores that were untreated (black square) or high vacuum-treated for 10 days (black triangle) were incubated on an LB medium agar pad at 37 °C and the percentages of germination and growth of ~500 individual spores was determined, all as described in methods. Sequential bright-field images of individual PS578 spores without e or with f a 10-day high vacuum exposure were also obtained at various times
