Table 1 Mean values and standard deviations of T1, Tlag, Trelease, and ΔTrelease for germination of B. subtilis PS533 untreated and high vacuum-treated spores with different germinantsa
From: Single-cell analysis reveals individual spore responses to simulated space vacuum
Treatment conditions | No. of spores examined (% spore germination) | T1 (min) | Tlag (min) | ΔTleakage (min) | ΔTrelease (min) | ΔTlys (min) |
|---|---|---|---|---|---|---|
1 mM L-valine (untreated) | 449 (99%) | 21.8 ± 19.2 | 23.0 ± 19.5 | 1.3 ± 0.9 | 5.0 ± 1.4 | 12.7 ± 4.5 |
1 mM L-valine vac 1 day | 467 (91%) | 26.5 ± 19.0 | 28.1 ± 17.6 | 1.6 ± 1.9 | 4.8 ± 1.8 | 16.1 ± 5.1 |
1 mM L-valine vac 10 day | 397 (84%) | 27.9 ± 17.1 | 29.6 ± 17.1 | 1.5 ± 1.8 | 4.9 ± 1.4 | 17.8 ± 9.5 |
AGFK (untreated) | 402 (93%) | 41.5 ± 21.1 | 42.5 ± 21.2 | 1.0 ± 0.5 | 4.6 ± 1.1 | 11.0 ± 4.3 |
10 mM AGFK vac 1 day | 416 (85%) | 43.5 ± 28.0 | 45.4 ± 28.2 | 2.0 ± 1.6 | 4.7 ± 1.0 | 15.0 ± 13.3 |
10 mM AGFK vac 10 day | 456 (78%) | 43.5 ± 26.2 | 45.0 ± 26.4 | 1.5 ± 1.0 | 4.5 ± 1.1 | 18.3 ± 6.5 |
CaDPA (untreated) | 299 (97%) | 30.4 ± 13.6 | 58.4 ± 20.0 | 28.0 ± 13.5 | 4.9 ± 1.3 | 25.5 ± 8.6 |
CaDPA vac 1 day | 437 (97%) | 35.5 ± 14.2 | 63.3 ± 17.5 | 27.8 ± 13.4 | 4.6 ± 1.6 | 26.4 ± 11.4 |
CaDPA vac 10 day | 464 (97%) | 39.4 ± 17.8 | 66.0 ± 21.2 | 26.6 ± 12.3 | 3.8 ± 1.3 | 29.6 ± 9.5 |
Dodecylamine (untreated) | 414 (99%) | 35.1 ± 23.3 | 37.2 ± 22.7 | 2.1 ± 2.8 | 4.1 ± ± 1.4 | 20.6 ± 18.7 |
Dodecylamine vac 1 day | 447 (87%) | 42.8 ± 25.2 | 49.8 ± 32.9 | 7.0 ± 13.2 | 4.6 ± 1.2 | 15.9 ± 12.4 |
Dodecylamine vac 10 day | 367 (82%) | 60.2 ± 34.8 | 72.6 ± 37.0 | 12.4 ± 15.5 | 4.7 ± 1.3 | 16.0 ± 9.7 |
