Fig. 1: Experimental schematic. | npj Microgravity

Fig. 1: Experimental schematic.

From: Modeling a potential SANS countermeasure by experimental manipulation of the translaminar pressure difference in mice

Fig. 1

To begin the experiment, surgery was performed to implant the infusion cannula and pressure-monitoring probe for future ICP elevation and measurement. Once animals had recovered, baseline measurements of IOP and ICP were taken, and the baseline TLPD was calculated. On the same day, the baseline OKR to measure contrast sensitivity was conducted. The next day, mice received saline injection in one eye (IOP control) and bead injection in the other eye (IOP elevation). The following day, ICP was raised via infusion of aCSF into the subarachnoid space. This infusion was maintained for the duration of the experiment. Beginning with the day of ICP elevation (the day after IOP elevation), experimental ICP and IOP were measured at defined intervals over the next 14 days and used to calculate the experimental TLPD. After 14 days, the experimental OKR to re-measure contrast sensitivity was conducted. The day after, animals were killed and their eyes and optic nerves collected for retinal immunofluorescence and transmission electron microscopy, respectively. The differences between the experimental and baseline values of ICP, IOP, and TLPD were used to calculate ΔICP, ΔIOP, and ΔTLPD.

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