Fig. 10: Tumorigenicity assay.

Cells were seeded on a soft agar to evaluate their tumorigenic potential. Five thousand cells/well of positive control cell line HT-1080 (a) and 67,000 cells/well of negative control cell line W138 (b) were used as control to validate the procedure. Twelve cells/well of MSCs were used for this assay. (c) Ground control and (d) microgravity were cells that were culture expanded for 1 week and (e) ground control and (f) microgravity were cells cultured for 2 weeks on Earth and in microgravity, respectively.