Fig. 2: Establishing MSC identity.

MSCs thawed from frozen BioCells after 1 week (a–d) and 2 weeks in culture at ISS were tripled stained with CD73, CD90, and CD105 antibodies and then analyzed using flow cytometry. a, c, e, g were from ground control samples. b, d, f, h were from ISS microgravity samples. R7 gate was used to select double positive for CD90 and CD73. R2 gate was applied out of R7 gate to demonstrate triple-positive cells for CD73, CD90, and CD105 (n = 3).