Fig. 3: Post-flight MSC proliferation analyses. | npj Microgravity

Fig. 3: Post-flight MSC proliferation analyses.

From: Feasibility, potency, and safety of growing human mesenchymal stem cells in space for clinical application

Fig. 3

a MSCs were cultured in triplicates after 1- or 2-week expansion in the ISS environment and on Earth in our laboratory. Cells were seeded into a 96-well plate and stained with IncuCyte Nuclight red to track cell proliferation by nucleus count. Real-time images were acquired by IncuCyte S3 at 2-h interval up to 48 h. Post flight, the ISS-expanded cells proliferated at comparable rate with ground control cells. Statistics determined by one-way ANOVA (n = 3). Error bars represent standard deviation (SD) (n = 3, P > 0.05). b Similarly, assessment of population doubling in longer-term cultures of 1-week ISS-expanded MSCs and the corresponding ground control showed no statistically significant difference in growth rate (two-sample Wilcoxon’s rank-sum (Mann–Whitney) test, P = 0.68). Error bars represent standard deviation (SD).

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