Fig. 4: Impact of microgravity on expression of cell cycle checkpoints associated genes. | npj Microgravity

Fig. 4: Impact of microgravity on expression of cell cycle checkpoints associated genes.

From: Feasibility, potency, and safety of growing human mesenchymal stem cells in space for clinical application

Fig. 4

MSCs were culture for 1 and 2 weeks. Cells were harvested and saved in RNAprotect. RNA was later isolated and quantitative reverse transcription PCR (RT-qPCR) analysis was performed as described in the “Methods” section. Expression of cell cycle checkpoint-associated genes: a cyclin-dependent kinase inhibitor 2A (CDKN2A), b transcription factor (E2F1), and c serine/threonine-protein kinase, known as polo-like kinase 1 (PLK1) were evaluated and compared to ground control gMSCs. Gene expression was normalized to GAPDH. Statistics determined by one-way ANOVA (n = 3, *P < 0.05), error bars represent standard deviation (SD).

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