Fig. 8: Genomic integrity analysis of the safety of growing MSC in space. | npj Microgravity

Fig. 8: Genomic integrity analysis of the safety of growing MSC in space.

From: Feasibility, potency, and safety of growing human mesenchymal stem cells in space for clinical application

Fig. 8

a Bone marrow-derived mesenchymal stem cells (5000 cells/well) previously grown for 1 week or 2 weeks in space, along with ground control cells were seeded to ibidi µ-Slide 8 Well. Cells were stimulated with 100 μM etoposide as indicated, fixed, and subjected to immunofluorescence analysis to visualize pS139-H2A.X. Scale bars indicate 10 μm. b shows quantitation of the fluorescence intensity of pS139-H2A.X (n = 40 cells). Analysis was conducted using the Image J software. Fluorescence intensities were normalized to the etoposide-treated group for each experimental condition. **** indicates statistical significance (<0.0001) determined by one-way ANOVA as compared to cells treated with 100 μM etoposide. Error bars represent standard deviation (SD).

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