Fig. 1: Recent JAXA space missions in Kibo of ISS.
a Cell culture system used in the Myo Lab project22. L6 rat myoblasts at 100% confluence in a disposable cultivation chamber (DCC) were fused by changing the medium to a differentiation medium (i.e., Dulbecco’s modified Eagle’s medium containing 0.5% fetal bovine serum). (Photo by Nikawa, T) b C. elegans culture system used in the CERISE project36,37,38. Upon reaching microgravity at KIBO, flight crews activated the experiments by removing the U-pin. Holder was set into Meas Exp Unit A and transferred into the Cell Biology Experiment Facility (CBEF) with or without 1-g rotation for either 4 or 8 days. (Photo by Higashitani, A). c Appearance of medaka reared during spaceflight. Medaka was filmed for abnormal behavior to examine physiological changes under microgravity. The recordings showed that the fish became accustomed to life under microgravity but displayed unique behavior such as swimming upside-down (red arrow). Scale bar, 10 mm. d Medaka chamber for live imaging used on the ISS. Twelve larvae at stage 39 can be kept in each “Medaka Chamber”, in which the larvae are placed in Mebiol Gel (Mebiol Inc., Kanagawa, Japan) and then covered with a gas-permeable membrane. The chamber shown was carried aboard Soyuz flight progress M-22M (54 P) (Roscosmos, Russia) in 2014. After arrival at the Japanese Experiment Module of the ISS, the chambers were set under a fluorescence microscope for live imaging for 8 days. The red dotted area indicates an enlarged view (inset) showing three larvae. Scale bar, 10 mm.
