Fig. 5: Representative confocal fluorescence micrographs.
From: Tracing transport of protein aggregates in microgravity versus unit gravity crystallization
a Lysozyme and b BSA crystals in the presence of 5% dimer of the respective protein labeled with Alexa Fluor 594 NHS ester grown at 1 G. Representative PfGST crystals were grown in the presence of c 1% tetramer impurity and d 5% tetramer impurity labeled with Alexa Fluor 488 TFP ester and the displayed crystals were also prepared under unit gravity conditions. The crystals from the capillaries utilized for further investigation were similar in size, which means ~180–220 µm in major axis length for PfGST, 80–120 × 80–120 × 250–300 µm for BSA and 120–180 µm in all three directions for lysozyme. Fluorescence analysis was performed on equal crystal volumes thereby eliminating the influence of crystal size on total fluorescence.
