Fig. 5: Unique genomic variants map to distinct regions of adapted genes.
From: Adaptation to simulated microgravity in Streptococcus mutans
a pknB acquired mutations that were unique to either simulated microgravity (green) or normal gravity (blue). Here we mapped all mutations detected (excluding nonsense mutations, as they lead to a premature stop codon which results in a shortened, and most often, nonfunctional protein product) at all four sequencing time points onto a homolog from Staphylococcus aureus (PDB 4EQM [https://www.rcsb.org/structure/4EQM])83 as there were no solved Streptococcus homologues in the database. b ptsH acquired the same SNP in both normal gravity and simulated microgravity (G54A/V-orange). This specific residue has been shown to be important for protein-protein interactions (PDB 1PTF [https://www.rcsb.org/structure/1PTF])89. c rex acquired a total of 12 unique variants in simulated microgravity (green), 5 unique variants in normal gravity (blue) with 3 variants in common (orange). Variants mapped onto the homologous Streptococcus agalactiae structure (PDB 3KET [https://www.rcsb.org/structure/3KET])99 show that they both interact directly with the DNA substrate. All structural figures were generated using The PyMOL™ Molecular Graphics System, Version 2.4.1, Schrödinger, LLC.
