Fig. 2: The WM-induced vacuole fusion assay can be carried out in the BRIC-PDFU. | npj Microgravity

Fig. 2: The WM-induced vacuole fusion assay can be carried out in the BRIC-PDFU.

From: Microgravity enhances the phenotype of Arabidopsis zigzag-1 and reduces the Wortmannin-induced vacuole fusion in root cells

Fig. 2

a Germination rate of WT and zig-1 seeds inside BRIC-PDFU during EVT. No significant difference in germination rate between two genotypes was detected after removing the two WT outliners (30% and 50%). (Unpaired t-test, ns: p > 0.05; n represents plate number, n > = 8). b Fixed root tip cortex cell vacuole morphology of WT and zig-1 (GFP-TIP2;1). Three-day-old WT and zig-1 seedlings were treated with DMSO buffer control or WM solution for 2 h and then seedlings were fixed and kept at 4 °C before confocal imaging. Scale bar: 20 μm. c Quantification of vacuole number in WT and zig-1 after chemical treatment. Cells from the cortex transition zone of the root tip were analyzed. (Two-way ANOVA, Tukey’s multiple comparison test, ****p < 0.0001; n represents cell number under analysis for each genotype with each treatment, n > 365). Raw datapoints are represented with filled circles. Error bars represent standard deviation.

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