Fig. 5: Bioprocessing system designs integrating selected methods for dewatering, lysis, and purification.
From: Theoretical design of a space bioprocessing system to produce recombinant proteins
a Design schematics with the following components: peristaltic pump (1), pinch valve (2), biomass reservoir (3), centrifuge cartridge (4), spent media reservoir (5), disposable bead beater (6), affinity resin reservoir (7), waste reservoir (8), wash buffer (9), elution buffer (10), product reservoir (11), tangential flow filter (12), crude lysate column (13), crude lysate chamber (14), affinity purification cartridge (15), affinity membrane (16), rollers (17). Yellow arrows indicate crew-assisted steps. b Flow diagram comparing the bioprocessing designs. Yellow boxes indicate operations that require crew-assistance to initiate or complete the operation.
