Fig. 6: Analysis of hFOB spheroid pressurization experiment on the ground.
From: Cellular mechanotransduction of human osteoblasts in microgravity
a Representative fluorescence images of hFOB spheroids encapsulated in collagen gels. On the ground, the spheroids were pre-treated with 100 ng ml−1 of BMP2 stimulation for 3 h (right) and subjected to 1 h of 0, 2, and 4 psi. Following BMP2 stimulation and application of compressive stress, the samples were frozen and subsequently fixed and immunostained for DAPI, F-actin, pSMAD1/5/9 and YAP. Scale bar is 100 µm. b Plots of relative fluorescence intensity of F-actin (left), pSMAD1/5/9 (middle), and YAP (right) for hFOB spheroids subjected to different levels of compressive stress and with (red) or without (gray) BMP2 stimulation. n = 9–18 spheroids per condition. *p < 0.05, **p < 0.01, ***p < 0.001.
