Fig. 2: General experimental scheme.

After 6 days of acclimation in microgravity, the maintenance media cassettes were switched to treatment media cassettes containing either maintenance media, 2% human serum, or 3 µM vitamin D binding protein (DBP) + 1.5 µM vitamin D (25(OH)D3) and treated for 2 days before fixation with either RNAlater for RNAseq or formalin for immunocytochemistry.