Table 1 Selected proteins from the whole proteome analysis of the simulated microgravity experiment compared to the control condition
From: Development and implementation of a simulated microgravity setup for edible cyanobacteria
MaGe IDs | UniProt IDs (BLAST similarity %) | Gene name | Product name | FC RPM 96 h | FC RPM 72 h |
|---|---|---|---|---|---|
Carbon fixation | |||||
ARTHROv5_50349 | H1WGH5 (100) | cbbS | Ribulose bisphosphate carboxylase, small chain (RuBisCO small subunit) | 1.43# | NS |
ARTHROv5_50351 | H1WGH7 (100) | cbbL | Ribulose bisphosphate carboxylase, large chain (RuBisCO large subunit) | 1.29# | 1.45# |
ARTHROv5_60384 | H1WI72 (100) | ccmM | Carboxysome assembly protein CcmM | 1.35# | 1.39# |
Glucose metabolism | |||||
ARTHROv5_10257 | H1WD12 (100) | putative UDP-glucose 4-epimerase | 0.29 | NS | |
ARTHROv5_10443 | H1WL88 (100) | pgi | Glucose-6-phosphate isomerase | 0.72 | NS |
ARTHROv5_10771 | H1WLS7 (99.71) | glpX | D-fructose 1,6-bisphosphatase class II | NS | 1.31# |
ARTHROv5_40290 | H1W658 (99.59) | tpiA | triosephosphate isomerase | 0.71# | NS |
ARTHROv5_50320 | H1WGE5 (100) | serA | D-3-phosphoglycerate dehydrogenase | 0.69# | 0.75# |
ARTHROv5_61108 | H1WF84 (100) | zwf | glucose-6-phosphate dehydrogenase | 0.59 | 0.69 |
Photosystems | |||||
ARTHROv5_10235 | H1WCZ0 (100) | psaC | Photosystem I iron-sulfur center (Photosystem I subunit VII) (9 kDa polypeptide) (PSI-C) (PsaC) | NS | 1.29# |
ARTHROv5_10245 | H1WCZ9 (100) | psbB | Photosystem II P680 chlorophyll A apoprotein (CP-47 protein) | NS | 1.31# |
ARTHROv5_10852 | H1WM00 (100) | psbP | Photosystem II oxygen-evolving complex 23 K protein | 1.41# | NS |
ARTHROv5_11973 | H1W800 (99.65) | btpA | Photosystem I biogenesis protein btpA | 2.18 | NS |
ARTHROv5_20093 | H1W8W7 (100) | psb27 | Photosystem II 11 kD protein (psb27) | 2.09 | NS |
ARTHROv5_30080 | H1W9J6 (100) | psaD | Photosystem I reaction center subunit II (Photosystem I 16 kDa polypeptide) (PSI-D) | 1.53 | 1.35# |
ARTHROv5_30570 | H1WAP8 (100) | psaE | Photosystem I reaction center subunit IV | 1.37# | NS |
ARTHROv5_30657 | H1WAX7 (100) | psaF | Photosystem I reaction center subunit III precursor (PSI-F) | 1.61 | 1.45# |
ARTHROv5_40153 | H1WC55 (100) | psbO | photosystem II manganese-stabilizing polypeptide precursor (MSP) | 1.28# | 1.32# |
ARTHROv5_40235 | H1W603 (100) | ycf37 | photosystem I assembly related protein Ycf37 | 5.04 | NS |
ARTHROv5_40969 | H1WDT4 (100) | psbU | Photosystem II 12 kDa extrinsic protein precursor (PS II complex 12 kDa extrinsic protein) (PSII-U) | 1.63 | NS |
ARTHROv5_41247 | H1WER4 (100) | ycf3 | Photosystem I assembly protein Ycf3 | 1.5# | NS |
ARTHROv5_50094 | H1WFR2 (100) | psbV | Cytochrome c-550 precursor (Cytochrome c550) (Low-potential cytochrome c) | 1.42# | NS |
ARTHROv5_60553 | H1WIK9 (100) | psbE | Cytochrome b559 subunit alpha (PSII reaction center subunit V) | 1.51 | 1.36# |
Phycobilisomes | |||||
ARTHROv5_11397 | H1W6S3 (100) | nblB2 | Phycocyanin alpha phycocyanobilin lyase related protein | 2.60 | NS |
ARTHROv5_10635 | H1WIF4 (100) | apcC | Phycobilisome 7.8 kDa linker polypeptide, allophycocyanin-associated, core (LC 7.8) | 1.58 | NS |
ARTHROv5_10637 | H1WLP4 (100) | apcA | Allophycocyanin alpha subunit | 1.52 | NS |
ARTHROv5_11553 | H1W701 (100) | cpcB | C-phycocyanin beta subunit | 1.45# | 1.42# |
ARTHROv5_11554 | H1W702 (100) | cpcA | C-phycocyanin alpha subunit | 1.59 | 1.36# |
ARTHROv5_11555 | H1W703 (100) | cpcC1 | Phycobilisome 32 kDa linker polypeptide, phycocyanin-associated, rod 1 | 1.89 | 1.75 |
ARTHROv5_11556 | H1W704 (99.65) | cpcC2 | Phycobilisome 32 kDa linker polypeptide, phycocyanin-associated, rod 2 | 1.22# | 1.28# |
ARTHROv5_11558 | H1W706 (100) | cpcE | Phycocyanin alpha subunit phycocyanobilin lyase | 3.33 | NS |
ARTHROv5_12132 | H1W8H4 (100) | apcF | Allophycocyanin beta-18 subunit | 2.13 | NS |
ARTHROv5_40726 | H1WB65 (100) | cpcG | phycobilisome rod-core linker protein | NS | 1.24# |
ARTHROv5_60056 | H1WGQ0 (100) | apcD | Allophycocyanin alpha-B subunit | 1.78 | NS |
ARTHROv5_61214 | H1WKH2 (100) | apcE | Allophycocyanin Phycobiliprotein | NS | 1.28# |
Electron transport chain and ATP synthesis | |||||
ARTHROv5_10430 | H1WLA1 (100) | petF | Ferredoxin-2 | 1.45# | NS |
ARTHROv5_12000 | H1W875 (100) | atpC | ATP synthase epsilon chain | 3.33 | NS |
ARTHROv5_12001 | H1W876 (100) | atpD | ATP synthase F1 complex subunit beta | 1.41# | 1.34# |
ARTHROv5_30365 | H1WA72 (100) | petD | Cytochrome b6-f complex subunit 4 (17 kDa polypeptide) | 1.35# | NS |
ARTHROv5_40277 | H1W646 (99.03) | petJ1 | cytochrome c6 | 1.40# | NS |
ARTHROv5_40850 | H1WD90 (100) | petA | Cytochrome f | NS | 1.41# |
ARTHROv5_41386 | H1WF80 (100) | petH | Ferredoxin–NADP reductase (FNR) | 1.27# | NS |
ARTHROv5_60533 | H1WIM8 (100) | atpG2 | ATP synthase B’ chain (Subunit II) | 2.22 | 1.64 |
ARTHROv5_60534 | H1WIM7 (100) | atpF | ATP synthase B chain (Subunit I) | 4.19 | 2.12 |
ARTHROv5_60535 | H1WIM6 (100) | atpH | ATP synthase delta chain; ATP synthase F1, delta subunit | 2.42 | 1.7 |
ARTHROv5_60536 | H1WIM5 (100) | atpA | F1 sector of membrane-bound ATP synthase, alpha subunit | 1.27# | 1.31# |
Nitrate uptake | |||||
ARTHROv5_11944 | H1W7Y5 (100) | nirA | ferredoxin-nitrite reductase | 0.35 | 0.42 |
ARTHROv5_40618 | H1W8M0 (100) | nrtD | ABC Nitrate transport system, ATP-binding component NrtD | 0.12 | 0.10 |
ARTHROv5_40619 | H1W8M1 (100) | nrtC | ABC Nitrate transport system, ATP-binding protein NrtC | 0.08 | 0.05 |
ARTHROv5_40620 | H1W8M2 (100) | nrtB | ABC Nitrate transport system, permease component NrtB | 0.06 | 0.09 |
ARTHROv5_40621 | H1W8M3 (100) | nrtA | ABC Nitrate transport system, periplasmic component NrtA | 0.29 | 0.27 |
Signaling | |||||
ARTHROv5_10210 | H1WCW8 (99.93) | Histidine kinase, putative two-component sensor | 3.35 | NS | |
ARTHROv5_11419 | Absent | ffh | signal recognition particle protein component | 0.64 | NS |
ARTHROv5_12124 | Absent | Histidine kinase | 0.75# | 0.79# | |
ARTHROv5_60977 | H1WK38 (100) | Histidine kinase, putative two-component sensor | 0.45 | 0.50 | |
Carotenoids | |||||
ARTHROv5_30214 | H1W9V6 (100) | Orange carotenoid-binding protein | 0.57 | NS | |
Ribosomal subunits | |||||
ARTHROv5_11031 | H1WMI3 (100) | rplC | 50S ribosomal subunit protein L3 | 0.63 | NS |
ARTHROv5_11032 | H1WMI2 (100) | rplD | 50S ribosomal subunit protein L4 | 0.76# | NS |
ARTHROv5_11038 | H1WMH6 (100) | rplP | 50S ribosomal subunit protein L16 | 0.67# | 0.58 |
ARTHROv5_11041 | H1WHN2 (100) | rplN | 50S ribosomal subunit protein L14 | NS | 0.73# |
ARTHROv5_11048 | H1WMG6 (100) | rplO | 50S ribosomal subunit protein L15 | 0.64 | NS |
ARTHROv5_11057 | H1WMF7 (100) | rplM | 50S ribosomal subunit protein L13 | 0.80# | 0.73# |
ARTHROv5_60158 | H1WH59 (100) | rpmA | 50S ribosomal subunit protein L27 | 0.80# | 0.73# |
ARTHROv5_60163 | H1WH86 (100) | rplU | 50S ribosomal protein L21 | 0.71# | 0.52 |
ARTHROv5_60949 | H1WH09 (100) | rplS | 50S ribosomal subunit protein L19 | 0.67# | NS |
ARTHROv5_60953 | Absent | rplA | 50S ribosomal subunit protein L1 | 0.74# | 0.74# |
ARTHROv5_60955 | H1WJZ2 (100) | rplL | 50S ribosomal subunit protein L7/L12 | 1.85 | NS |
Gas vesicles | |||||
ARTHROv5_12033 | G9BN15 (100) | gvpC1 | Gas vesicle structural protein GvpC (short variant) | 2.29 | NS |
ARTHROv5_12037 | G9BMZ1 (100) | gvpC | Gas vesicle structural protein GvpC | 1.60 | NS |
ARTHROv5_20211 | H1W967 (100) | gvpFL | Protein GvpF/L | 1.43# | NS |
