Fig. 2: Adherence of VVE-B (vancomycin-variable E. faecium) isolates tested with crystal violet (0.5%) biofilm assay (n = 7). | npj Microgravity

Fig. 2: Adherence of VVE-B (vancomycin-variable E. faecium) isolates tested with crystal violet (0.5%) biofilm assay (n = 7).

From: Systematic screening of 42 vancomycin-resistant Enterococcus faecium strains for resistance, biofilm, and desiccation in simulated microgravity

Fig. 2

Initial: adherent cell formation was initially evaluated with the crystal violet assay before any treatment. 1 g: isolates were incubated in normal gravity as a control for 7 days. Sim. µg: isolates were incubated under sim. µg for 7 days by clinorotation. Isolates were then tested in their ability to adhere and potentially form biofilms after 24 h at 37 °C in measuring the optical density (OD600nm) in a microplate reader. Measurements were done in triplicates and calculated error bars show the standard deviation of each sample (n = 3). Significance was determined by two-sample t-test; the conditions corresponding to the significance levels are shown in the parentheses: **p < 0.01; ***p < 0.001.

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