Fig. 2: THP-1 cell adhesion to HUVEC layer.
From: Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight
Representative images (a) before and (b) after floating THP-1 cells adhere to the HUVEC layer (arrow). Scale bar: 50 µm. c The number of newly adhering THP-1 cells to the HUVEC layer within each parabola of the partial gravity phases (0.16 g and 0.38 g) and in 1 g assessed by video analysis (cell number/FOV (field of view)). Bar charts represent mean values ± SD and symbols represent number of cells/FOV. d Total number of THP-1 cells adhering to HUVEC layer assessed by confocal microscopic assessment (cell number/FOV). Inflight fixation of samples was performed after P8 and numbers were compared to baseline collection on ground (1 g). Boxes indicate median and interquartile range; whiskers represent minimum and maximum (three FOV/slide; two slides per condition for 0.16 g and 1 g and one slide per condition for 0.38 g). *P < 0.05, **P < 0.01, ***P < 0.001.
