Fig. 4: F-actin organization in HUVEC cells.
From: Lunar and Martian gravity alter immune cell interactions with endothelia in parabolic flight
Fixation of cells was performed either before start of parabolas (inflight control without flow, 1 g) or after eight parabolas in lunar (0.16 g) or Martian (0.38 g) gravity, respectively. a Overview of representative images of F-Actin by confocal microscopy. Roundish structures on the F-actin network are adherent THP-1 cells. b thickness, (c) angle, (d) anisotropy of F-actin. Thick dash lines in violin plots represent mean values and thin dash lines represent quartiles. *P < .05, **P < 0.01, ***P < 0.001 (n = 1 for 0.38 g and n = 2 for 0.16 g; three FOVs per slide analyzed).
