Fig. 5: Adv-BMP9 infection of primary osteocytes alleviates the osteocytic transcriptional phenotype and cell acidification induced by high concentrations of TGFβ1.
From: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling
A Phosphorylation of Smads in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, as detected by western blot. B BMP-pSmad1/5/8 signaling target gene expression in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 (n = 3). C Intracellular pH changes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1, normalized to control group. D Expression of osteoclastic genes in primary osteocytes following infection with Adv-BMP9 or control virus in the presence of 10 ng/ml TGFβ1 (n = 3). ns no significance, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
