Fig. 6: Adv-BMP9 inhibits osteoclastogenesis, suppresses adipogenic differentiation of marrow stromal cells, and promotes chondrogenic differentiation.
From: BMP9 attenuates microgravity-related disuse osteoporosis by modulating TGFβ and BMP signaling
A Osteoclastogenesis assay: TRAP staining and bovine bone slice osteoclast resorption assay, Scale bar: Black/White 50 μm. B TRAP-positive cell count and osteoclast count (n = 3). C Osteoclastogenesis gene expression analysis (n = 3). D Alizarin Blue staining for chondrogenic differentiation of marrow stromal cells after 7 days of induction. E Chondrogenic differentiation gene expression in marrow stromal cells (n = 3). F Adipogenic differentiation assay: bright field/ Oil Red O staining of marrow stromal cells after 7 days of induction. G Adipogenic differentiation-related gene expression in marrow stromal cells (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
