Fig. 4: Vasoconstriction induced by flexible electronic delivery of PEFs.
a, b view from the top of the device placed on the vascularized tumor and a zoom on the region of interest at the beginning (a) and the end (b) of the experiment. Texas Red dextran was injected into the vascular circulation to visualize blood vessels with fluorescence microscopy at λexc = 595 nm. White arrows show the location of the measure, performed between the dotted lines depicting the edges of the blood vessel at the beginning (yellow) and the end (blue) of the experiment. Scale bars: (a, b) left panel: 400 µm, right panel: 100 µm. b Normalized diameter over time after PEFs delivery (5 pulses–20 V–100 µs–1 Hz) depending on the blood vessel diameter. The pulse train is represented in blue. Negative control: N = 3, large blood vessels: N = 6, medium blood vessels: N = 11, small blood vessels: N = 3. Error bars indicate standard deviations.
