Fig. 6: Uptake of PI due to PEFs.
a Spheroids observed at λexc = 550 nm before and after application of PEFs. b Normalized fluorescence intensity of calcium signals (λexc = 490 nm) measured over time of spheroids subjected to 10 trains of PEFs (red ticks). Trains of 5 pulses (20 V–100 µs–1 Hz) are delivered every 30 s. c, d Two-photon maximum intensity projections of implanted spheroids at (c) control and (d) pulsed states (five 20 V pulses with 100 µs pulse width at 1 Hz, repeated 10 times at 30 s interstimulus intervals). e Zoom-in to the region marked with the square on d. f–g Raw (f) and normalized (g) side projection pixel intensity profile of propidium-iodide staining from control spheroid (blue) and after pulsing (red). FWHM: full width at half maximum. Scale bars: 100 µm.
