Table 2 Chemical composition of 1L ATCC1249 medium.
Component I | MgSO4 | 2.0 g |
Na3C6H5O7 | 5.0 g | |
CaSO4 | 1.0 g | |
NH4Cl | 1.0 g | |
Distilled water | 400 ml | |
Component II | K2HPO4 | 0.5 g |
Distilled water | 200 ml | |
Component III | NaC3H5O3 | 3.5 g |
Yeast extract | 1.0 g | |
Distilled water | 400 ml | |
Component IV | Filter-sterilized 5 wt. % (NH4)2Fe(SO4)2 Add 0.1 ml of this solution to 5.0 ml of medium prior to inoculation |