Fig. 4 | npj Parkinson's Disease

Fig. 4

From: Mitochondrial impairment in microglia amplifies NLRP3 inflammasome proinflammatory signaling in cell culture and animal models of Parkinson’s disease

Fig. 4

Mitochondrial superoxide generation plays a role in pesticide-induced NLRP3 inflammasome activation. a ICC showing MitoSox generation from pesticide-treated primed microglial cells. Lower panel shows interactive 3D surface plot from corresponding ICC, which shows the intensity of superoxide generation. Scale bar, 100 μm. b ICC showing co-localization of mitochondrial superoxide generation and NLRP3. The right-most panel is the representative 3D color plot. Mito-apocynin reduced this co-localization between NLRP3 and MitoSox. Scale bar, 15 μm. c–d Luminex assays demonstrate that mito-apocynin reduced IL-1β secretion from primed microglial cells treated with rotenone c and tebufenpyrad d. e MitoSox assays reveal that mito-apocynin reduced pesticide-induced superoxide generation in pesticide-treated primed microglial cells. Data analyzed via two-way ANOVA with Bonferroni adjustment, *p < 0.05, **p < 0.01, ***p < 0.001 and are represented as Mean ± SEM with n = 3–8

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