Fig. 2

Electrophysiology on VGlut1 and VGlut2 striatal afferents. a Illustration in the sagittal plane demonstrates the placement of CAV2-Cre and AAV2-ChR2 injections into CAV2^Cre-Slc17a6^+/+ controls and CAV2^Cre-Slc17a6^lox/lox mice. b Representative traces show that light activation of VGLUT2 thalamostriatal synapses produces opsin-generated eEPSCs in SPNs from CAV2^Cre-Slc17a6^+/+ controls n = 5; left) but not in CAV2^Cre-Slc17a6^lox/lox mice (n = 9; center). Amplitude of light activated eEPSCs in SPNs from CAV2^Cre-Slc17a6^+/+ controls and CAV2^Cre-Slc17a6^lox/lox mice (right). c Bipolar stimulation of thalamostriatal projections produced eEPSCs in SPNs from CAV2^Cre-Slc17a6^+/+ controls (n = 5; left) but not in CAV2^Cre-Slc17a6^lox/lox mice (n = 4; center). Amplitude of bipolar activated eEPSCs in SPNs from CAV2^Cre-Slc17a6^+/+ controls and CAV2^Cre-Slc17a6^lox/lox mice (right). For c and d, synaptic currents were eliminated after adding NBQX to the bath solution, indicating that they arose from activation of glutamatergic receptors (red traces). d Bipolar stimulation of corticostriatal projections produced eEPSCs in SPNs from both CAV2^Cre-Slc17a6^+/+ controls (n = 5; left) and CAV2^Cre-Slc17a6^lox/lox mice (n = 5; center). Amplitude of bipolar activated eEPSCs in SPNs from CAV2^Cre-Slc17a6^+/+ controls and CAV2^Cre-Slc17a6^lox/lox mice (right). Data in all figures are shown as mean ± standard error of mean (SEM)