Fig. 5
From: Altered dopamine release and monoamine transporters in Vps35 p.D620N knock-in mice
Extracellular striatal dopamine in VKI animals. a Placement of the probe (coordinates AP +0.5, ML +2.1, DV −2.8) for in-vivo detection of dopamine in the dorsolateral striatum. (Schematic adapted from Franklin, K.B.J. & Paxinos, G. The Mouse Brain in Stereotaxic Coordinates (copyright: Academic Press, an imprint of Elsevier, Amsterdam, 2013).40) b Average levels from four dialysates collected over an hour, every 15 min. No difference was found in overall basal levels of dopamine (1-way ANOVA F2,28 = 0.61, p = 0.54). c Dopamine metabolites total levels (DOPAC (i) and HVA (ii) and their ratio over levels of dopamine (iii). Homo VKI mice show similar, albeit towards increased, levels of DOPAC and HVA (i and ii, 1-way ANOVA F2,28 = 2.89, p = 0.07 and F2,28 = 3.06, p = 0.06, respectively), with a significantly higher ratio indicating greater turnover of dopamine (iii; 1-way ANOVA F2,28 = 6.44, p < 0.04; Bonferroni post-test WT vs Het t(28) = 0.40, p = 0.99, WT vs Homo t(28) = 3.20, p = 0.03)
