Fig. 5
High expression levels of Wt, but not A30P, a-syn shift the distribution of REs toward the plasma membrane. RBL cells were co-transfected with mCh-Rab11 to label REs, and low or high levels of EGFP or Wt a-syn, or high levels of A30P a-syn. a Confocal micrographs of mCh-Rab11 fluorescence (REs) co-transfected with low levels (5 μg) of Wt a-syn or high levels (25 μg) of Wt or A30P a-syn. The fluorescence intensity within a thin shell around the plasma membrane (dashed lines) divided by total cell fluorescence was used to calculate % membrane proximal REs; scale bar = 10 μm. b Averaged % membrane proximal REs for specified samples in a box plot; number of cells evaluated for each condition is specified. The box represents 25th–75th percentile of the data, the midline represents the median and the small square represents the average. ***P-values < 0.001; NS, not statistically significant (P-values > 0.05)
