Table 1 Functional effects and structural interactions of Wt a-syn and selected mutants
Conditions | Wt-a-syn | A30P-a-syn | V70P-a-syn | |||
|---|---|---|---|---|---|---|
Stimulated exocytosis—low expression (Fig. 2f) | ↓+++ | ↓+++ | – | |||
Stimulated exocytosis—high expression (Fig. 2h) | ↑+ | ↓+++ | ↑+ | |||
Binding to mitochondria—no stress (Fig. 6) | + | – | – | |||
Binding to mitochondria—with stress (Fig. 6) | +++ | + | – | |||
Binding to lipid droplets—high expression levels (Fig. S6) | +++ | – | NT | |||
Helix1 | Helix2 | Helix1 | Helix2 | Helix1 | Helix2 | |
– | – | + | +++ | – | +++ | |
Disruption of secondary structure (micelles, NMR) (Fig. 3) | Helix1 | Helix2 | Helix1 | Helix2 | Helix1 | Helix2 |
– | – | + | – | – | + | |
