Fig. 1 | npj Parkinson's Disease

Fig. 1

From: Infection with mosquito-borne alphavirus induces selective loss of dopaminergic neurons, neuroinflammation and widespread protein aggregation

Fig. 1

Regional specificity of recombinant Western equine encephalitis virus following intranasal infection. a Schematic diagram illustrating the structure of vectors for each recombinant virus used in these studies. Subgenomic promoter (SPG), untranslated region (UTR). b Schematic diagram illustrating treatment regimen with WEEV. c Raw image of luciferase activity in McFly-infected mice (left-24 HPI, middle-48 HPI, right-72 HPI). d Pseudo-colored image of luciferase activity overlaid onto whole body images during the timecourse of infection. e Ex vivo sagittal hemispheric images of luciferase activity at specific times post infection demonstrating progressive spread of WEEV from the olfactory bulb to caudal brain regions. f Six-week-old CD-1 mice were administered 1 × 104 PFU of DsRed producing WEEV (McRed) via intranasal inoculation and euthanized 4 days post infection. Brains were cryosectioned coronally and dsRed + infected nuclei were labeled (purple) with reference to the allen brain atlas. Individual nuclei with presence of Dsred were labeled: GL (glomerular layer), SI (substantia innominate), MB (mammillary hippocampus (HIP), thalamus (TH), entorhinal regions of the cortex (ENT), and pons

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