Fig. 7: FRET response to pilot HTS hits on intra-protomeric αSN biosensor.
From: Potent inhibitors of toxic alpha-synuclein identified via cellular time-resolved FRET biosensors
Hits that demonstrated robust, reproducible FRET response in the inter-protomeric biosensor CRC were then evaluated with the intra-protomeric αSN biosensor. With both donor and acceptor XFP fused to one construct, this biosensor is sensitive to both oligomer formation as well as conformation. Three hits reduced the intra-protomeric FRET (RO, BAY, DEM) significantly, as did known αSN modulator EGCG. Experiments were performed with n = 3; *p < 0.05, **p < 0.01, ***p < 0.001, ns indicates no significant difference as determined by Student’s t test.
