Fig. 2: SIRT2 expression remains unchanged in PD models.

a The mRNA levels of SIRT2 in the SNpc were examined using RT-PCR in mice treated with normal saline (NS) or MPTP. b Quantification of SIRT2 mRNA levels shown in (a) (n = 3). c The protein levels of SIRT2 in the SNpc were examined using western blotting in mice treated with NS or MPTP. d Quantification of SIRT2 protein levels shown in (c) (n = 3). e–h mRNA and protein levels of SIRT2 in the SNpc assessed were assessed using RT-PCR and western blotting (n = 3), respectively, in WT and α-synuclein-A30P*A53T transgenic (TG) mice. i–l mRNA and protein levels of SIRT2 were examined in primary culture neurons treated with 50 μM MPP⁺ for 0, 6, 12, and 24 h using RT-PCR and western blotting (n = 3). m–p SIRT2 expression was examined in SY5Y cells stably expressing GFP vector, GFP-α-synuclein, GFP-α-synuclein-A53T, and GFP-α-synuclein-A30P*A53T (n = 3). All data are presented as the means ± SD. Statistical analyses were conducted using an unpaired t-test in (b) and (d). Statistical analyses were conducted using two-way ANOVA followed by Tukey’s post hoc test in (f) and (h). Statistical analyses were conducted using one-way ANOVA followed by Tukey’s post hoc test in (j, l, n, p). None of the differences were significant.