Fig. 4: Nuclear localization of SIRT2 promotes neuronal death.

a Primary culture neurons were transiently transfected with GFP vector or GFP-NLS-SIRT2 plasmids and subsequently stained using anti-SIRT2 antibodies (green), propidium iodide (PI) (red), and DAPI (blue). The scale bar represents 10 μm. b GFP-positive cells (green) that also showed positive PI staining (red) were counted as dead neurons. The percentage of PI-positive cells (red) among at least 100 GFP-positive cells was measured for each group (n = 3). All data are presented as the means ± SD. Statistical analyses were conducted using one-way ANOVA followed by Tukey’s post hoc test, and ** represents P < 0.01. c HEK293 cells were transfected with GFP vector and GFP-NLS-SIRT2 plasmids for 24 h and subsequently collected for RNA-seq. The differentially expressed genes between the two groups are illustrated using a heatmap (n = 3). The colors indicate relative expression levels (red, high expression; green, low expression). d Gene Ontology (GO) terms. Frequency of sequences with assigned GO terms for the biological process category across the different samples. e KEGG pathway enrichment. Biological pathways associated with the differentially expressed genes in the different samples.