Table 1 Antibodies.
From: DAT and TH expression marks human Parkinson’s disease in peripheral immune cells
Specificity | Clone/species | Conjugate | Vendor | Catalog Number | Purpose | Dilution | Concentration |
|---|---|---|---|---|---|---|---|
TH | Polyclonal/Rabbit | N/A | Sigma | AB152 | FC | 1:100 | 0.01 mg/mL |
DAT | MAB369/Rat | N/A | Sigma | MAB369 | FC | 1:100 | 0.01 mg/mL |
DAT | Polyclonal/Rabbit | N/A | Sigma | AB5802 | FC | 1:200 | 0.02 mg/mL |
Rabbit | Polyclonal/Goat | BV421 | BD | 565014 | FC | 1:40 | 0.005 mg/mL |
Rat | Polyclonal/Goat | APC | BD | 551019 | FC | 1:40 | 0.005 mg/mL |
CD14 | MfP9/Mouse | FITC | BD | 347493 | FC | 1:50 | 0.0005 mg/mL |
CD14 | M5E2/Mouse | PE | Biolegend | 301805 | FC | 1:200 | 0.004 mg/mL |
CD15 | W6D3/Mouse | BV605 | Biolegend | 323031 | FC | 1:100 | 0.008 mg/mL |
CD11b | ICRF44/Mouse | BV421 | Biolegend | 301323 | FC | 1:50 | 0.016 mg/mL |
CD11b | M170/Rat | FITC | Biolegend | 101205 | FC | 1:100 | 0.05 mg/mL |
CD16 | 3G8/Mouse | APC | Biolegend | 302011 | FC | 1:50 | 0.016 mg/mL |
HLA-DR | L243/Mouse | APC-Cy7 | Biolegend | 307617 | FC | 1:50 | 0.016 mg/mL |
Live/Dead | N/A | ZombieRed | Biolegend | 423109 | FC/Utility | 1:1000 | N/A |
Ly6C | HK1.4/Rat | BV785 | Biolegend | 128041 | FC | 1:200 | 0.001 mg/mL |
Ly6G | 1A8/Rat | PE | Biolegend | 127607 | FC | 1:100 | 0.002 mg/mL |
TH | Polyclonal/Chicken | AF647 | Encor | CPCA-TH | FC | 1:45 | 0.01 mg/mL |
TH | MCA-4H2/Mouse | N/A | Encor | MCA-4H2 | IHC | 1:500 | 0.002 mg/mL |
IBA1 | Polyclonal/Rabbit | N/A | Wako | 019–19741 | IHC | 1:500 | 0.001 mg/mL |
NET | Monoclonal/Mouse | N/A | MAB Tech | NET17–1 | FC | 1:100 | 0.005 mg/mL |
Mouse | Polyclonal/Goat | HRP | Biolegend | 405306 | IHC | 1:250 | 0.004 mg/mL |
Rabbit | Polyclonal/Donkey | HRP | Biolegend | 406401 | IHC | 1:250 | 0.004 mg/mL |
