Table 2 Reagents and materials.

Ficoll-Paque Plus

GE

45–001–750

PBMC isolation

N/A

PBS

In house

N/A

PBMC isolation, FC

1x

K2EDTA Vacutainer

BD

366643

Blood collection

N/A

Butterfly blood collection device

BD

367342

Blood collection

N/A

L-DOPA

Sigma

D1507

IP Injection (mouse)

6.25 mg/kg

Benserazide

Sigma

B7283

IP Injection (mouse)

10 mg/kg

FACS tubes

Fisher

FC, mouse PBMC isolation

N/A

6-OH-DA

Tocris

2547

IC injection (mouse)

5ug/uL

MPTP

Sigma

M0896

Injection (mouse)

TritonX-100

ThermoFisher

BP151–100

IHC

0.5%

Goat serum

Lampire Biologicals

7332500

IHC

5%

Leucosep Tubes

Grenier BioOne

227290 P

PBMC isolation

N/A

Trypan Blue

MP Biomedicals

1691049

Cell counting

Stock

Fix/Perm Kit

eBioscience

88–8824–00

FC

Stock

Anti-TH

Encor

CPCA-TH

FC

1ug/mL

Leucosep Tube

Grenier BioOne

227290 P

PBMC isolation

N/A

Diet Gel

ClearH2O

76 A

Mouse diet supplement

Stock

Bilateral Cannula

P1

C235IS-5/SP

6-OH-DA infusion

N/A

Infusion tubing

P1

8F023X041P01

6-OH-DA infusion

N/A

Syringe

Exel

26016

IP injection, cardiac puncture blood draw

N/A

Isoflurane

Patterson

07–893–8441

Anesthesia

1–5%

Permount

Fisher

SP15–500

IHC

Stock

DAB substrate

Sigma

D4293

IHC

Stock

SG substrate

Vector Labs

VK4700

IHC

Stock

PFA

Sigma

158127

IHC

4%

AlexaFluor 647 labeling reagent

ThermoFisher

A20173

FC conjugation

Stock

Filtration column

Biorad

7322010

Affinity purification

N/A