Table 1 Characterization of non-modified aSyn antibodies using recombinant synuclein proteins and peptides.
Antibody | Epitope | Species reactivity | bSyn detection | gSyn detection | Other amyloidogenic protein reactivity | Neighboring PTM sensitivity | Conformational selectivity | |
|---|---|---|---|---|---|---|---|---|
WB | DB | |||||||
LASH-EGT403 | 1–5 | hu, mus | + | + | – | – | m/o/f | m/o/f |
5B10-A12 | 1–10 | hu, mus | – | – | – |
| m/o/f | m/o/f |
LASH-EGTNter | 1–20 | hu, mus | + | – | – | – | m/o/f | m/o/f |
LASH-BL 34–45 | 34–45 | hu, mus | + | – | – |
| m/f | m/o/f |
LASH-BL 80–96 | 80–96 | hu, mus | – | – | – | – | m/o/f | m/o/f |
BD SYN-1 | 91–99 | hu, mus | – | – | – | – | m/o/f | m/o/f |
BL 4B12 | 103–108 | hu | – | – | – | – | m/o/f | m/o/f |
2F10-E12 | 110–115 | hu, mus | – | – | – | – | m/o/f | m/o/f |
AB LB509 | 115–122 | hu | – | – | – |
| m/o/f | m/o/f |
7H10-E12 | 115–125 | hu | – | – | – | pY125 | m/o/f | m/o/f |
LASH-BL 117–122 | 117–122 | hu | – | – | – |
| m/o/f | m/o/f |
LASH-BL A15127A | 120–122 | hu | – | – | – | – | m/o/f | m/o/f |
4E9-G10 | 120–125 | hu | – | – | – | pY125 | m/o/f | m/o/f |
1F10-B12 | 121–125 | hu | – | – | – | – | m/o/f | m/o/f |
4E9-C12 | 121–132 | hu | – | – | – | pY125 | m/o/f | m/o/f |
2C4-B12 | 123–125 | hu | – | – | – | pY125 | m/o/f | m/o/f |
6B2-D12 | 126–132 | hu, mus | – | – | +/TDP-43 | pS129 | m/o/f | m/o/f |
AB 134–138 | 134–138 | hu, mus | + | – | – |
| m/o/f | m/o/f |
M5
Y39
P120-Y125
P120-Y125
D135
 = truncation, aSyn alpha-synuclein, bSyn beta-synuclein, DB dot blot, f fibril, gSyn gamma-synuclein, hu human, m monomer, mus mouse, o oligomer, PTM post-translational modification, TDP-43 transactive response DNA-binding protein 43 kDa, WB Western blot.
 = truncation, aSyn alpha-synuclein, bSyn beta-synuclein, DB dot blot, f fibril, gSyn gamma-synuclein, hu human, m monomer, mus mouse, o oligomer, PTM post-translational modification, TDP-43 transactive response DNA-binding protein 43 kDa, WB Western blot.