Table 2 Specificity summary of aSyn antibodies on aSyn KO mouse neuronal culture and brain tissues.

Antibody	Epitope	Specificity: aSyn KO neurons - ICC	Specificity: aSyn KO neurons—WB		Specificity: aSyn KO tissue—IHC
Antibody	Epitope	Specificity: aSyn KO neurons - ICC	15 kDa	35-180 kDa	Specificity: aSyn KO tissue—IHC
LASH-EGT403	1–5	+	+	–	+
5B10-A12	1–10	+	+	–	+
LASH-EGTNter	1–20	+/bSyn-positive	+	–	+
LASH-BL 34–45	34–45	+/bSyn-positive	+	–	+
LASH-BL 80–96	80–96	−/hipp aSyn KO only	+	–	+
BD SYN-1	91–99	+	+	+	+
BL 4B12	103–108	−/mild nuclear	+	–	+
2F10-E12	110–115	+	+	–	+
AB LB509	115–122	+	+	–	+
7H10-E12	115–125	–	+	+	+
LASH-BL 117–122	117–122	–	+	+	+
LASH-BL A15127A	120–122	–	+	–	+
4E9-G10	120–125	+	+	–	+
1F10-B12	121–125	–	+	+	+
4E9-C12	121–132	+	+	+	+
2C4-B12	123–125	–	+	+	+
6B2-D12	126–132	+	+	–	+
AB 134–138	134–138	+/bSyn-positive	+/bSyn-positive	–	+
LASH-BL pY39	pY39	+	+	–	+
LASH-EGT pY39	pY39	+	+	–	+
LASH pS87	pS87	−/mild nuclear	+	+	−/punctate cytoplasmic
LASH-EGT pY125	pY125	+	+	+	−/punctate cytoplasmic
AB pY125	pY125	+	+	+	+
AB EP1536Y	pS129	+	+	+	+
LASH-EGT pS129	pS129	+	–	–	−/punctate cytoplasmic
AB pY133	pY133	+	+	+	+
AB pY136	pY136	+	+	+	+
LASH-EGT nY39	nY39	+	+	+	+
5E1-G8	nY39	–	+	–	−/mild diffuse background
5E1-C10	nY39	–	+	–	−/mild diffuse background
6A3-E9	aSyn-120	+	+	+	+

+ specific, − non-specific, aSyn alpha-synuclein, bSyn beta-synuclein, hipp hippocampus, ICC immunocytochemistry, IHC immunohistochemistry, kDa kilodalton, KO knockout, WB Western blot.

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