Table 4 A summary of the mouse aSyn-reactive antibody detection patterns of exogenous PFFs in aSyn KO neurons, newly formed aggregates in PFF-seeded WT neurons and mouse brain tissues.
Antibody | Epitope | aSyn KO neurons | PFF seeding model: WT primary neurons | PFF seeding model: WT mouse brain tissue | |||
|---|---|---|---|---|---|---|---|
PFF detection - ICC | PFF detection - WB | Positivity | aSyn pS129* overlap | Positivity | aSyn pS129* overlap | ||
LASH-EGT403 | 1–5 | + | + | + | – | – | – |
5B10-A12 | 1–10 | + | + | + | + | + | + |
LASH-EGTNter | 1–20 | + | + | + | + | + | + |
LASH-BL 34–45 | 34–45 | + | + | + | + | + | – |
LASH-BL 80–96 | 80–96 | + | + | + | – | + | – |
BD SYN-1 | 91–99 | + | + | + | – | + | – |
2F10-E12 | 110–115 | + | + | + | – | + | + |
6B2-D12 | 126–132 | + | – | + | – | + | – |
AB 134–138 | 134–138 | + | + | + | + | + | + |
LASH-EGT pY39 | pY39 | – | – | + | – | + | – |
AB EP1536Y | pS129 | – | – | + | + | + | + |
LASH-EGT pS129 | pS129 | – | – | + | + | + | + |
AB pY133 | pY133 | – | – | + | – | – | – |
AB pY136 | pY136 | – | – | + | – | – | – |
LASH-EGT nY39 | nY39 | – | – | – | – | – | – |
5E1-G8 | nY39 | + (non-specific) | – | + (non-specific) | – | – | – |
5E1-C10 | nY39 | + (non-specific) | – | + (non-specific) | – | – | – |
