Fig. 1: Analysis of LRRK2 and mtDNA damage in HEK293 cells lines stably overexpressing either WT-LRRK2 or G2019S-LRRK2.

a Representative western blot of WT-LRRK2 or G2019S-LRRK2 overexpressing cells show expression of LRRK2 pSer1292 and full-length LRRK2. β-actin was blotted as a loading control. b Quantification of western blots demonstrate ~6-fold increase of LRRK2 pSer1292 in G2019S-LRRK2 compared to WT-LRRK2 expressing cells. c Representative western blots of WT-LRRK2 or G2019S-LRRK2 overexpressing cells show expression of LRRK2 pSer935 and full-length LRRK2. β-actin was blotted as a loading control. d Quantification of western blots demonstrate no difference of LRRK2 pSer935 levels between G2019S-LRRK2 and WT-LRRK2 expressing cells. e Quantification of western blots demonstrate no difference of LRRK2 protein levels between the two cell lines. f Mitochondrial DNA damage was increased in G2019S-LRRK2 relative to WT-LRRK2 expressing cells. g The differences in mtDNA damage between the cell lines were not attributable to changes in steady state mtDNA levels. Data are presented as mean ± SEM. n = 3–4 replicates. Statistical significance was determined by unpaired t-test for all analyses. GS-LRRK2 G2019S-LRRK2, WT-LRRK2 wild-type LRRK2, ns non-significant.